We describe a label-free approach based on Raman spectroscopy, to study drug-induced apoptosis in vivo. Spectral-shifts at wavenumbers associated with DNA, proteins, lipids, and collagen have been identified on breast and melanoma tumor tissues. These findings may enable a new analytical method for rapid readout of drug-therapy with miniaturized probes.
Characterization of magnetic nanoparticle-seeded microspheres for magnetomotive and multimodal imaging
Magnetic iron-oxide nanoparticles have been developed as contrast agents in magnetic resonance imaging (MRI) and as therapeutic agents in magnetic hyperthermia. They have also recently been demonstrated as contrast and elastography agents in magnetomotive optical coherence tomography and elastography (MM-OCT and MM-OCE, respectively). Protein-shell microspheres containing suspensions of these magnetic nanoparticles in lipid cores, and with functionalized outer shells for specific targeting, have also been demonstrated as efficient contrast agents for imaging modalities such as MM-OCT and MRI, and can be easily modified for other modalities such as ultrasound, fluorescence, and luminescence imaging. In addition to multimodal contrast-enhanced imaging, these microspheres could serve as drug carriers for targeted delivery under image guidance. Although the preparation and surface modifications of protein microspheres containing iron oxide nanoparticles has been previously described and feasibility studies conducted, many questions regarding their production and properties remain. Since the use of multifunctional microspheres could have high clinical relevance, here we report a detailed characterization of their properties and behavior in different environments to highlight their versatility. The work presented here is an effort for the development and optimization of nanoparticle-based microspheres as multi-modal contrast agents that can bridge imaging modalities on different size scales.
The Arnold and Mabel Beckman Foundation announced today its 2017 class of Arnold O. Beckman Postdoctoral Fellows, individuals who underscore the Foundation’s mission of supporting basic research in the chemistry and life sciences. They were selected after a three-part review led by a panel of scientific experts.
The Foundation will award more than $2.6 million in funding for 20 exceptional research fellows from 13 universities
Chemicals like nitric oxide and hydrogen peroxide can promote cancer growth. MPC-CMSE Summer Scholar Kaila Holloway is working in the lab of Michael S. Strano, Carbon P. Dubbs Professor in Chemical Engineering at MIT, to develop tiny chemical sensors to detect their concentrations near tumors in the body.
MIT News – Melanie Miller Kaufman – Department of Chemical Engineering – April 24, 2017
Chemical engineering and chemistry postdocs “expected to become the next generation of leaders and innovators in science, engineering, and technology.”
Danielle Mai and Freddy Nguyen from the MIT Department of Chemical Engineering, along with Liela Bayeh and Julianne Troiano of the Department of Chemistry, were awarded 2017 Arnold O. Beckman Postdoctoral Fellowships. The two-year, competitive program will support each researcher’s continuing work in their corresponding labs.
Freddy Nguyen, a member of the Michael Strano lab, is working to develop nanoscale molecular sensors for probing cancer tumors and their microenvironments. He would like to implant nanosensors inside tumors to measure their response, at the molecular level, to various cancer therapies such as chemotherapeutics and radiation therapy. In 2016, he earned his medical degree from the University of Illinois at Chicago, and in 2015 received a PhD in physical chemistry from the University of Illinois at Urbana-Champaign.
Arnold and Mabel Beckman Foundation – March 30, 2017
2017 Beckman Postdoctoral Fellow
Massachusetts Institute of Technology
Research: Development of nanosensors for in-vivo monitoring of cancer therapeutics
2015 PhD graduate awarded Beckman Postdoc Fellowship – March 30, 2017
Congratulations to Freddy Nguyen, a 2015 Illinois Chemistry PhD graduate, who was chosen for a prestigious Arnold O. Beckman Postdoctoral Fellows Award. Nguyen is a postdoctoral researcher at MIT working on development of nanosensors for in vivomonitoring of cancer therapeutics.
According to Nguyen, “The research I am planning to pursue is focused on the development of nanoscale molecular sensors for probing the tumor and its microenvironment. More specifically, we would like to implant our nanosensors inside tumors to to measure their response at the molecular level to various cancer therapies such as chemotherapeutics and radiation therapy. Our nanosensors are detected using near-infrared fluorescence and Raman spectroscopic techniques allowing us to probe the sensors from a distance using near-infrared light and are not susceptible to photobleaching effects unlike typical endogenous and exogenous fluorophores. These unique features of our nanosensors can allow us with a method to dynamically probe the tumor microenvironment in real-time and in-vivo. Patients currently have to wait until there are measurable size changes on CT or MRI scans or must undergo biopsies of the tumor to determine molecular changes in response to treatment. Having access to that molecularinformation within the first few days of treatment will be a tremendous step forward indetermining whether cancer treatments are working for each patient at a much earlier timeframe than the current standard of care. This allows for the patient and physician to morepromptly manage the treatment of their cancer.”
Investigating Effects of Proteasome Inhibitor on Multiple Myeloma Cells Using Confocal Raman Microscopy
Due to its label-free and non-destructive nature, applications of Raman spectroscopic imaging in monitoring therapeutic responses at the cellular level are growing. We have recently developed a high-speed confocal Raman microscopy system to image living biological specimens with high spatial resolution and sensitivity. In the present study, we have applied this system to monitor the effects of Bortezomib, a proteasome inhibitor drug, on multiple myeloma cells. Cluster imaging followed by spectral profiling suggest major differences in the nuclear and cytoplasmic contents of cells due to drug treatment that can be monitored with Raman spectroscopy. Spectra were also acquired from group of cells and feasibility of discrimination among treated and untreated cells using principal component analysis (PCA) was accessed. Findings support the feasibility of Raman technologies as an alternate, novel method for monitoring live cell dynamics with minimal external perturbation.