Freddy T. Nguyen, MD, PhD

Transfusion Medicine Fellow @ Dartmouth-Hitchcock Medical Center

Research Fellow @ Massachusetts Institute of Technology

Physician-scientist with extensive experience developing and translating nanotechnologies and biomedical optical technologies from the bench to clinic in areas of genetics, oncology, and cardiovascular diseases. Extensive leadership experience in community building in healthcare innovation, research, medical, and physician-scientist communities.

Color-blind fluorescence detection for four-color DNA sequencing

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Ernest K. Lewis, Wade C. Haaland, Freddy T. Nguyen, Daniel A. Heller, Matthew J. Allen, Robert R. MacGregor, C. Scott Berger, Britain Willingham, Lori A. Burns, Graham B.I. Scott, Carter Kittrell, Bruce R. Johnson, Robert F. Curl, Michael L. Metzker. Proceedings of the National Academy of Sciences 2005-02-28
We present an approach called pulsed multiline excitation (PME) for measurements of multicomponent, fluorescence species and demonstrate its application in capillary electrophoresis for DNA sequencing. To fully demonstrate the advantages of PME, a fluorescent dye set has been developed whose absorption maxima span virtually the entire visible spectrum. Unlike emission wavelength-dependent approaches for identifying fluorescent species, the removal of the spectral component in PME confers a number of advantages including higher and normalized signals from all dyes present in the assay, the elimination of spectral cross-talk between dyes, and higher signal collection efficiency. Base-calling is unambiguously determined once dye mobility corrections are made. These advantages translate into significantly enhanced signal quality as illustrated in the primary DNA sequencing data and provide a means for achieving accurate base-calling at lower reagent concentrations.
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