In vivo detection of drug-induced apoptosis in tumors using Raman spectroscopy.

TitleIn vivo detection of drug-induced apoptosis in tumors using Raman spectroscopy.
Publication TypeJournal Article
Year of Publication2018
AuthorsJonas, Oliver, Kang Jeon Woong, Singh Surya P., Lammers Alex, Nguyen Freddy T., Dasari Ramachandra R., So Peter T. C., Langer Robert, and Cima Michael J.
JournalAnalyst
Volume143
Issue20
Pagination4836-4839
Date Published2018 Oct 08
ISSN1364-5528
KeywordsAnimals, Antibodies, Antineoplastic Agents, Apoptosis, Breast Neoplasms, Caspase 3, Doxorubicin, Immunohistochemistry, Intercalating Agents, Melanoma, Mice, Nude, Spectrum Analysis, Raman
Abstract

We describe a label-free approach based on Raman spectroscopy, to study drug-induced apoptosis in vivo. Spectral-shifts at wavenumbers associated with DNA, proteins, lipids, and collagen have been identified on breast and melanoma tumor tissues. These findings may enable a new analytical method for rapid readout of drug-therapy with miniaturized probes.

DOI10.1039/c8an00913a
Alternate JournalAnalyst
PubMed ID30070266
PubMed Central IDPMC6175619
Grant ListP41 EB015871 / EB / NIBIB NIH HHS / United States
R37 CA224144 / CA / NCI NIH HHS / United States