Investigating Effects of Proteasome Inhibitor on Multiple Myeloma Cells Using Confocal Raman Microscopy.

TitleInvestigating Effects of Proteasome Inhibitor on Multiple Myeloma Cells Using Confocal Raman Microscopy.
Publication TypeJournal Article
Year of Publication2016
AuthorsKang, Jeon Woong, Singh Surya P., Nguyen Freddy T., Lue Niyom, Sung Yongjin, So Peter T. C., and Dasari Ramachandra R.
JournalSensors (Basel)
Volume16
Issue12
Date Published2016 Dec 14
ISSN1424-8220
Abstract

Due to its label-free and non-destructive nature, applications of Raman spectroscopic imaging in monitoring therapeutic responses at the cellular level are growing. We have recently developed a high-speed confocal Raman microscopy system to image living biological specimens with high spatial resolution and sensitivity. In the present study, we have applied this system to monitor the effects of Bortezomib, a proteasome inhibitor drug, on multiple myeloma cells. Cluster imaging followed by spectral profiling suggest major differences in the nuclear and cytoplasmic contents of cells due to drug treatment that can be monitored with Raman spectroscopy. Spectra were also acquired from group of cells and feasibility of discrimination among treated and untreated cells using principal component analysis (PCA) was accessed. Findings support the feasibility of Raman technologies as an alternate, novel method for monitoring live cell dynamics with minimal external perturbation.

DOI10.3390/s16122133
Alternate JournalSensors (Basel)
PubMed ID27983660
PubMed Central IDPMC5191113
Grant ListP41 EB015871 / EB / NIBIB NIH HHS / United States
R01 HL121386 / HL / NHLBI NIH HHS / United States
R21 NS091982 / NS / NINDS NIH HHS / United States
U01 NS090438 / NS / NINDS NIH HHS / United States