High resolution live cell Raman imaging using subcellular organelle-targeting SERS-sensitive gold nanoparticles with highly narrow intra-nanogap.

TitleHigh resolution live cell Raman imaging using subcellular organelle-targeting SERS-sensitive gold nanoparticles with highly narrow intra-nanogap.
Publication TypeJournal Article
Year of Publication2015
AuthorsKang, Jeon Woong, So Peter T. C., Dasari Ramachandra R., and Lim Dong-Kwon
JournalNano Lett
Volume15
Issue3
Pagination1766-72
Date Published2015 Mar 11
ISSN1530-6992
KeywordsCell Line, Tumor, Contrast Media, Gold, Humans, Image Enhancement, Microscopy, Mouth Neoplasms, Nanoparticles, Particle Size, Reproducibility of Results, Sensitivity and Specificity, Spectrum Analysis, Raman, Subcellular Fractions
Abstract

We report a method to achieve high speed and high resolution live cell Raman images using small spherical gold nanoparticles with highly narrow intra-nanogap structures responding to NIR excitation (785 nm) and high-speed confocal Raman microscopy. The three different Raman-active molecules placed in the narrow intra-nanogap showed a strong and uniform Raman intensity in solution even under transient exposure time (10 ms) and low input power of incident laser (200 μW), which lead to obtain high-resolution single cell image within 30 s without inducing significant cell damage. The high resolution Raman image showed the distributions of gold nanoparticles for their targeted sites such as cytoplasm, mitochondria, or nucleus. The high speed Raman-based live cell imaging allowed us to monitor rapidly changing cell morphologies during cell death induced by the addition of highly toxic KCN solution to cells. These results strongly suggest that the use of SERS-active nanoparticle can greatly improve the current temporal resolution and image quality of Raman-based cell images enough to obtain the detailed cell dynamics and/or the responses of cells to potential drug molecules.

DOI10.1021/nl504444w
Alternate JournalNano Lett.
PubMed ID25646716
PubMed Central IDPMC4356631
Grant List9P41EB015871-27 / EB / NIBIB NIH HHS / United States
R01 EY017656 / EY / NEI NIH HHS / United States
5R01NS051320 / NS / NINDS NIH HHS / United States
4R44EB012415-02 / EB / NIBIB NIH HHS / United States
R01 NS051320 / NS / NINDS NIH HHS / United States
R44 EB012415 / EB / NIBIB NIH HHS / United States
5R01EY017656-02 / EY / NEI NIH HHS / United States
P41 EB015871 / EB / NIBIB NIH HHS / United States