Measuring uptake dynamics of multiple identifiable carbon nanotube species via high-speed confocal Raman imaging of live cells.

TitleMeasuring uptake dynamics of multiple identifiable carbon nanotube species via high-speed confocal Raman imaging of live cells.
Publication TypeJournal Article
Year of Publication2012
AuthorsKang, Jeon Woong, Nguyen Freddy T., Lue Niyom, Dasari Ramachandra R., and Heller Daniel A.
JournalNano Lett
Volume12
Issue12
Pagination6170-4
Date Published2012 Dec 12
ISSN1530-6992
KeywordsAnimals, Biological Transport, Cell Line, Macrophages, Mice, Microscopy, Confocal, Molecular Imaging, Nanotubes, Carbon, Spectrum Analysis, Raman
Abstract

Carbon nanotube uptake was measured via high-speed confocal Raman imaging in live cells. Spatial and temporal tracking of two cell-intrinsic and nine nanotube-derived Raman bands was conducted simultaneously in RAW 264.7 macrophages. Movies resolved single (n, m) species, defects, and aggregation states of nanotubes transiently as well as the cell position, denoted by lipid and protein signals. This work portends the real-time molecular imaging of live cells and tissues using Raman spectroscopy, affording multiplexing and complete photostability.

DOI10.1021/nl302991y
Alternate JournalNano Lett.
PubMed ID23151070
PubMed Central IDPMC3561480
Grant ListP41 EB015871 / EB / NIBIB NIH HHS / United States
P41EB015871-26A1 / EB / NIBIB NIH HHS / United States