Interferometric synthetic aperture microscopy.

TitleInterferometric synthetic aperture microscopy.
Publication TypeJournal Article
Year of Publication2007
AuthorsRalston, Tyler S., Marks Daniel L., P Carney Scott, and Boppart Stephen A.
JournalNat Phys
Volume3
Issue2
Pagination129-134
Date Published2007 Feb 01
ISSN1745-2473
Abstract

State-of-the-art methods in high-resolution three-dimensional optical microscopy require that the focus be scanned through the entire region of interest. However, an analysis of the physics of the light-sample interaction reveals that the Fourier-space coverage is independent of depth. Here we show that, by solving the inverse scattering problem for interference microscopy, computed reconstruction yields volumes with a resolution in all planes that is equivalent to the resolution achieved only at the focal plane for conventional high-resolution microscopy. In short, the entire illuminated volume has spatially invariant resolution, thus eliminating the compromise between resolution and depth of field. We describe and demonstrate a novel computational image-formation technique called interferometric synthetic aperture microscopy (ISAM). ISAM has the potential to broadly impact real-time three-dimensional microscopy and analysis in the fields of cell and tumour biology, as well as in clinical diagnosis where imaging is preferable to biopsy.

DOI10.1038/nphys514
Alternate JournalNat Phys
PubMed ID25635181
PubMed Central IDPMC4308056
Grant ListR01 EB005221 / EB / NIBIB NIH HHS / United States
R21 EB005321 / EB / NIBIB NIH HHS / United States