Interferometric synthetic aperture microscopy.

TitleInterferometric synthetic aperture microscopy.
Publication TypeJournal Article
Year of Publication2007
AuthorsRalston, Tyler S., Marks Daniel L., P Carney Scott, and Boppart Stephen A.
JournalNat Phys
Date Published2007 Feb 01

State-of-the-art methods in high-resolution three-dimensional optical microscopy require that the focus be scanned through the entire region of interest. However, an analysis of the physics of the light-sample interaction reveals that the Fourier-space coverage is independent of depth. Here we show that, by solving the inverse scattering problem for interference microscopy, computed reconstruction yields volumes with a resolution in all planes that is equivalent to the resolution achieved only at the focal plane for conventional high-resolution microscopy. In short, the entire illuminated volume has spatially invariant resolution, thus eliminating the compromise between resolution and depth of field. We describe and demonstrate a novel computational image-formation technique called interferometric synthetic aperture microscopy (ISAM). ISAM has the potential to broadly impact real-time three-dimensional microscopy and analysis in the fields of cell and tumour biology, as well as in clinical diagnosis where imaging is preferable to biopsy.

Alternate JournalNat Phys
PubMed ID25635181
PubMed Central IDPMC4308056
Grant ListR01 EB005221 / EB / NIBIB NIH HHS / United States
R21 EB005321 / EB / NIBIB NIH HHS / United States